Graduate Education Institute · Food Engineering · Master
ECTS: 7.5 T+P+L: 3+0+0 Departmental Elective
Coordinator: Doç. Dr. Banu METİN
Course Objective
To gain information on advances in molecular food microbiology and implement the techniques learnt to specific microorganisms
Course Content
DNA, RNA and protein basics, isolation of nucleic acids and visualization using agarose gel electrophoresis, polymerase chain reaction (PCR), real time PCR and sequence analysis, molecular identification of microorganisms, typing and phylogenetics
Required Resources
Thieman WJ ve Palladino MA (2013) Biyoteknolojiye Giriş. Çeviri Editörü: Mücella Tekeoğlu. Palme Yayıncılık
Buckingham L (2019) (Molecular Diagnostics: Fundamentals, Methods, and Clinical Applications
Recommended Resources
Cadotte MW, Davies TJ (2016) Phylogenies in Ecology: A Guide to Concepts and Methods, Princeton University Press
Course Learning Outcomes
- DNA, RNA ve proteinlerle ilgili temel bilgilerin kazanılması
- Nükleik asit izolasyon teknikleri hakkında bilgi sahibi olmak
- Agaroz jel elektroforezi, PCR ve real time PCR hakkında bilgi sahibi olmak
- PCR'da kullanılabilecek primer tasarımı yapabilmek
- Dizilimlerin analizi ile ilgili yöntemler hakkında bilgi sahibi olmak
- Değişik mikroorganizmaların moleküler yöntemlerle nasıl tanımlanabileceğinin öğrenilmesi
- Mikroorganizma tiplendirme yöntemleri hakkında bilgi sahibi olmak
Core Area Distribution
(42) Life Sciences%40 (44) Physical Sciences%40 (52) Engineering and Engineering Trades%20
Teaching Methods
ExpressionQuestion-AnswerDiscussionExercise and PracticeSelf studyProject Based Learning (Including Field Work)
Assessment & Evaluation
HomeworkPerformance Assignment ( Lab / Workshop / Field Work / Seminar / Presentation / Completion Study / ThesisTesting (Essay / Tests: True-Falls, multiple-choice, short answer, matching)
ECTS / Workload
| Activity | Quantity | Duration (h) | Total Workload |
|---|---|---|---|
| Course Duration (Including Exam Week) | 15 | 3 | 45 |
| Out of Class Study Period | 15 | 8 | 120 |
| Midterm | 0 | 0 | 0 |
| Quiz | 0 | 0 | 0 |
| Assignment | 6 | 3 | 18 |
| Practice | 1 | 1 | 1 |
| Final | 1 | 1 | 1 |
Course Schedule
| Week | Subject | Preparation |
|---|---|---|
| 1 | Molecular Microbiology Introduction | Lecture notes related section |
| 2 | DNA, RNA and proteins | Lecture notes related section |
| 3 | Control of gene expression and mutations | Lecture notes related section |
| 4 | Nucleic acid purification methods | Lecture notes related section |
| 5 | Agarose gel electrophoresis and analysis of nucleic acids | Lecture notes related section |
| 6 | Ploymerase chain reaction (PCR), real time PCR, NCBI database and primer design | Lecture notes related section |
| 7 | DNA sequencing, visualization of sequences, comparison of sequences to the databases, BLAST), sequence alignment | Lecture notes related section |
| 8 | Molecular identification of bacteria | Lecture notes related section |
| 9 | Molecular Identification of Fungi | Lecture notes related section |
| 10 | Construction of phylogenetic trees | Lecture notes related section |
| 11 | Typing of microorganisms | Lecture notes related section |
| 12 | Investigation of microbial diversity | Lecture notes related section |
| 13 | Project presentations | Studies conducted throughout the semestre |
| 14 | Project presentations | Studies conducted throughout the semestre |
| 15 | Final | Final |