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MBG 103 - Molecular Biology Introduction

Faculty of Engineering and Natural Sciences · Molecular Biology and Genetics (English 30%) · Undergraduate

ECTS: 7 T+P+L: 3+0+3 Compulsory
Coordinator: Dr. Öğr. Üyesi Mehmetali TİBATAN

Course Objective

Aim of this course;

  • Introduction to Molecular Biology course aims to prepare students for the fundamental concepts and terminology of the molecular biology discipline.
  • While doing so, it also aims to teach general methods in molecular biology, such as RNA-DNA isolation and PCR techniques, and to explain and familiarize students with their working principles and mechanisms.
  • In addition to the basic concepts of molecular biology, teaching the detailed function and structure of the DNA is among the course objectives.
  • The importance of central dogma in the fundemental functions of nucleic acids inthe cell will also be covered within this course.
  • The course objectives also include teaching about experiments conducted in molecular biology, the types of model organisms used in these experiments, and the reasons for selecting these types.

Course Content

The course includes:

  • General concepts; basic information such as DNA-RNA, nucleotide differences in nucleic acids,
  • Use of micropipettes in molecular biology methods, nucleic acid isolation and PCR technique
  • Types and structures of biomolecules (carbohydrates, fats, protein structure and the structure of nucleic acids),
  • The concept of central dogma and its components,
  • The basic structure of proteins and protein folding properties,
  • The concept of model organisms used in experiments.

Required Resources

Campell Biology (12th edition) by Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece

Recommended Resources

Fundamental Molecular Biology, 3rd Edition Lizabeth A. Allison

Explanations

Midterm: 25%

Lab: 25%

Final:50%

Rules

  • All students are required to attend 70% of the theoretical lectures and 80% of the laboratory lectures.
  • Students with a laboratory grade point below 50 points will not be allowed to take the final exam.
  • The passing grade for the course is 50 points.
  • The allowed time for being late to class is the first 10 minutes.
  • The allowed time for being late to the exam is 20 minutes.
  • If anyone leaves the exam within the first 20 minutes, late students will not be admitted regardless of the 20-minute time tolerance.
  • Non-class activities (playing games, making phone calls, phone texting, consuming strongly scented foods such as spices, garlic, and onions, personal care, etc.) are prohibited during class.

Course Learning Outcomes

  1. They will be able to understand the jargon used in the disciplines of molecular biology and genetics and acquire the necessary background.
  2. They will learn the pathway from DNA to protein and be will be able to explain the connection between them.
  3. Understand molecular mechanism inside the cell
  4. Learn to the regulation of gene expression
  5. Have knowledge about the latest developments and application areas in Molecular Biology and Genetics
  6. They will be able to learn about the 3D structure and folding features of proteins.
  7. Able to repeat the basic steps and application of the PCR technique in a laboratory setting.
  8. Able to use and calibrate a micropipette.
  9. They will learn concept of model organism and important experiments for the molecular biology in the science history
  10. Will gain knowledge about how enzymes work
  11. Will be able to explain detailed 3D structure and molecular structure of the DNA

Core Area Distribution

(42) Life Sciences%80 (72) Health%20

Teaching Methods

ExpressionQuestion-AnswerExercise and PracticeGuided PracticeExperiment - Test / Lab/ Workshop / Field Practice

Assessment & Evaluation

Performance Assignment ( Lab / Workshop / Field Work / Seminar / Presentation / Completion Study / ThesisTesting (Essay / Tests: True-Falls, multiple-choice, short answer, matching)

ECTS / Workload

ActivityQuantityDuration (h)Total Workload
Course Duration (Including Exam Week)15345
Out of Class Study Period15345
Midterm122
Quiz000
Assignment000
Practice13339
Final122

Course Schedule

WeekSubjectPreparation
1Introduction to the courseCourse content will be shared
2General conceptCourse content will be shared
3Biomolecules I; Lipids, CarbohydratesCourse content will be shared
4Biomolecules II; Proteins-nucleic bases-energy moleculesCourse content will be shared
5Nucleic AcidsCourse content will be shared
6Proteins and folding propertiesCourse content will be shared
7MidtermMidterm
8Enzymes-1Course content will be shared
9Enzymes-IICourse content will be shared
10Enzymes II continue- Enzyme inhibition typesCourse content will be shared
11Central dogmaCourse material will be shared
12Central dogma-continiuumCourse content will be shared
13Model organism conceptCourse content will be shared
14Important experiments in molecular biologyCourse content will be shared
15General repeats and exam preparationsCourse content will be shared
16Final ExamFinal Exam