Mühendislik ve Doğa Bilimleri Fakültesi · Moleküler Biyoloji ve Genetik (%30 İngilizce) · Lisans
Dersin Amacı
The aim of this course is to provide students with a comprehensive foundation in the principles and applications of recombinant DNA technology.
Ders İçeriği
This course introduces the fundamental principles and applications of recombinant DNA technology. Students will learn the key tools and techniques of molecular cloning, sequencing, and genome editing, with applications in medicine, agriculture, and industry. The course also explores ethical and biosafety considerations.
Zorunlu Kaynaklar
Gene Cloning and DNA Analysis, 8th Ed., TA Brown, 2021 John Wiley & Sons Ltd.
Önerilen Kaynaklar
1. Principles of Gene Manipulation, 6th Ed., Sandy B. Primrose, Richard M Twyman & Robert W Old.
2. Molecular biotechnology: principles and applications of recombinant DNA, 4th Ed., Bernard R. Glick, Jack J. Pasternak, and Cheryl L. Patten.
Açıklamalar
1. A midterm exam will be held during the semester, and a comprehensive final exam will be held at the end of the semester.
2. Three of four short quizzes and one or two assignments (not exceeding five in total) will be given during the semester.
3. The dates for the short quizzes and assignments will be announced to students during classes.
Kurallar
Students are required to comply with and follow the rules and ethical principles regarding course attendance, exams, and assignments as specified in the IZU regulations.
Dersin Öğrenme Çıktıları
- Will explain the fundamental principles and applications of recombinant DNA technology.
- Will explain the principles of DNA purification, manipulation, and molecular analysis.
- Will analyze the structure, selection, and utilization of various cloning and expression vectors.
- Will evaluate host cell selection and the mechanisms of DNA transformation and uptake.
- Will explain the principles and applications of PCR and DNA amplification techniques.
- Will explain the principles of DNA sequencing and sequencing-based DNA analysis.
- Will describe the construction and applications of genomic and cDNA libraries.
- Will explain gene expression systems and approaches for studying gene function in prokaryotic and eukaryotic organisms.
- Will differentiate methodologies for large-scale analysis of genomes, transcriptomes, and proteomes.
- Will describe modern genome editing technologies and their specialized applications.
- Will appraise real-world applications of gene cloning and DNA analysis in medical diagnosis, therapy, and agricultural biotechnology.
Temel Alan Dağılımı
Öğretim Yöntem ve Teknikleri
Ölçme ve Değerlendirme
AKTS / İş Yükü
| Etkinlik | Sayı | Süre (saat) | Toplam İş Yükü |
|---|---|---|---|
| Ders Süresi (Sınav Haftası Dahil) | 15 | 3 | 45 |
| Sınıf Dışı Ders Çalışma Süresi | 15 | 2 | 30 |
| Ara Sınav | 1 | 10 | 10 |
| Kısa Sınav | 4 | 5 | 20 |
| Ödev | 1 | 5 | 5 |
| Uygulama | 0 | 0 | 0 |
| Final | 1 | 15 | 15 |
Ders Akışı
| Hafta | Konu | Ön Hazırlık |
|---|---|---|
| 1 | Introduction to Recombinant DNA Technology | Brown Ch. 1 |
| 2 | Purification and Manipulation of DNA | Brown Ch. 3, 4 |
| 3 | Cloning and Expression Vectors | Brown Ch. 2, 6, 7 |
| 4 | Host Cells & Transformation Methods | Brown Ch. 5 |
| 5 | PCR and DNA Amplification | Brown Ch. 9 |
| 6 | DNA Sequencing & Analysis | Brown Ch. 10 |
| 7 | Ara Sınav | Ara Sınav |
| 8 | Gene Expression and Function | Brown Ch. 11 |
| 9 | Studying Genomes, Transcriptomes and Proteoms | Brown Ch. 12, 13 |
| 10 | Studying Genomes, Transcriptomes and Proteoms | Brown Ch. 12, 13 |
| 11 | Genome Editing Technologies | Course materials will be shared |
| 12 | Recombinant Protein Production | Brown Ch. 14 |
| 13 | Gene Cloning and DNA Analysis in Medicine | Brown Ch. 15 |
| 14 | Gene Cloning and DNA Analysis in Agriculture | Brown Ch.16 |
| 15 | Preparation for the Final Exam | Overall Reviewing of the course topics |
| 16 | Final | Final |