Mühendislik ve Doğa Bilimleri Fakültesi · Moleküler Biyoloji ve Genetik (%30 İngilizce) · Lisans
Dersin Amacı
The aim of this course is to provide an in-depth exploration of the molecular mechanisms governing the gene expression, moving beyond basic inheritance to focus on complex regulatory networks and epigenetic control.
Ders İçeriği
Molecular Genetics II is the advanced continuation of Molecular Genetics I course. While MG I focused on the central dogma, macromolecular structures, and core molecular mechanisms, this course expands into regulation, maintenance, and manipulation of genetic information. Topics include gene regulation in prokaryotes and eukaryotes, epigenetics, RNA-based regulation, DNA repair, mobile genetic elements, genome organization, and modern genome editing technologies.
Zorunlu Kaynaklar
1. Anthony J. F. Griffiths et al., Introduction to Genetic Analysis, 12th Edition, Macmillan Learning, 2020
2. Harvey Lodish et al., Molecular Cell Biology, 8th Edition, W.H. Freeman, 2016
Önerilen Kaynaklar
Bruce Alberts et al. Essential Cell Biology, W. W. Norton, 2023
Açıklamalar
1. A midterm exam will be held during the semester, and a comprehensive final exam will be held at the end of the semester.
2. Five short quizzes or assignments (not exceeding five in total) will be given during the semester.
3. The dates for the short quizzes and assignments will be announced to students during classes.
Kurallar
Students are required to comply with and follow the rules and ethical principles regarding course attendance, exams, and assignments as specified in the IZU regulations.
Dersin Öğrenme Çıktıları
- Explain general principles of the central dogma and basic genomic studies
- Describe chromatin structure, epigenetic modifications, and their effects on gene activity
- Understand the molecular regulation of gene expression in prokaryotic and eukaryotic systems
- Analyze pos-transcriptional and post-translational regulation mechanisms
- Explain DNA damage, repair pathways, and their biological significance
- Understand the principles of genetic recombination and double-strand break repair system
- Evaluate the effects of transposons, and their molecular significance in genomic analysis
- Understand the developments in functional genomic studies
- Evaluate the molecular basis of oncogenesis and tumor supressor genes
- Gain a conceptual overview of modern genomic tools to prepare for Recombinant DNA Technology
Temel Alan Dağılımı
Öğretim Yöntem ve Teknikleri
Ölçme ve Değerlendirme
AKTS / İş Yükü
| Etkinlik | Sayı | Süre (saat) | Toplam İş Yükü |
|---|---|---|---|
| Ders Süresi (Sınav Haftası Dahil) | 15 | 3 | 45 |
| Sınıf Dışı Ders Çalışma Süresi | 15 | 2 | 30 |
| Ara Sınav | 1 | 10 | 10 |
| Kısa Sınav | 5 | 4 | 20 |
| Ödev | 0 | 0 | 0 |
| Uygulama | 0 | 0 | 0 |
| Final | 1 | 20 | 20 |
Ders Akışı
| Hafta | Konu | Ön Hazırlık |
|---|---|---|
| 1 | Introduction and Central Dogma Recap | Griffiths Ch. 7, 8, 9, 12.1, Lodish Ch. 5.2 - 5.4, 9.4 |
| 2 | Genome Organization and Chromatin Structure | Griffiths Ch. 12.2, 12.3, 12.4, Lodish Ch. 8.1, 8.2, 8.5, 8.6, 9.7 |
| 3 | Regulation of Gene Expression in Prokaryotes (1) | Griffiths Ch. 11, Lodish Ch. 9 |
| 4 | Regulation of Gene Expression in Prokaryotes (2) Regulation of Gene Expression in Eukaryotes (1) | Griffiths Ch. 11 and 12, Lodish Ch. 9 |
| 5 | Regulation of Gene Expression in Eukaryotes (2) | Griffiths Ch. 12, Lodish Ch. 9 |
| 6 | Post-Transcriptional (RNA-Based) and post-translational Regulation | Griffiths Ch. 12, Lodish Ch. 10.1-10.4 |
| 7 | Ara Sınav | Ara Sınav |
| 8 | Genome Integrity: DNA Damage and Specialized Repair Mechanisms | Griffiths Ch. 15, Lodish Ch. 5.6 |
| 9 | Genetic Recombination and Double-Strand Break Repair | Griffiths Ch. 15 |
| 10 | Mobile Genetic Elements | Griffiths Ch. 16, Lodish Ch. 8.3 |
| 11 | Functional Genomics | Griffiths Ch. 14.7, Lodish Ch. 6.2, 6.3, 8.4 |
| 12 | Molecular Cancer | Griffiths Ch. 13.7, Lodish Ch. 24 |
| 13 | Genome Editing Technologies | Griffiths Ch. 10 |
| 14 | Examples of current molecular genetics research | Article reading |
| 15 | General Review | Overviewing and getting ready for the final exam |
| 16 | Final Sınavı | Final Sınavı |